Multiple Forms of Nuclear Ribonuclease H from Tetrahymena pyriformis
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چکیده
منابع مشابه
Reactivation of Glycerinated Cilia from Tetrahymena Pyriformis
The ease with which Tetrahymena can be grown in relatively large quantity makes it an exceptionally useful source of cilia for biochemical study. Several methods of isolating the cilia have been described (3, 11). Although excellent for many purposes, these procedures all suffer from the limitation that the isolated cilia seem incapable of being reactivated by ATP. This paper describes a method...
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Frayed cilia from Tetrahymena pyriformis have been examined in the electron microscope using both uranyl acetate and phosphotungstic acid as negative stains. Observations on the central microtubules show that one member of the pair exhibits two rows of short projections. These are about 18 nm long, and are spaced longitudinally at 16-nm intervals. In many specimens the projections are separated...
متن کاملMitochondrial and Cytoplasmic Ribosomes from Tetrahymena Pyriformis
Mitochondrial and cytoplasmic ribosomes from Tetrahymena pyriformis have been isolated and studied by the techniques of polyacrylamide gel electrophoresis and electron microscopy used in conjunction. Although the two ribosome types show the same coefficient of sedimentation (80S) in sucrose gradients, they can be distinguished by gel electrophoresis: mitoribosomes migrate in a single band, cons...
متن کاملStudies on Nuclear Structure and Function in Tetrahymena Pyriformis
This report describes a rapid, efficient method for isolating macronuclei from Tetrahymena. The macronuclear fraction contains only small amounts of micronuclear material and little detectable whole cell or cytoplasmic contamination. A method is also described for preparing a "micronuclear fraction" which contains 20-40 micronuclei for every macronucleus present. Electron microscope observation...
متن کاملActivity of Ribonuclease, Acid Phosphatase, and Phosphodiesterase in Tetrahymena Pyriformis during Growth
Studies on the nucleolytic activity of cell homogenates of Tetrahymena pyriformis indicated that enzyme activity was, among other factors, a function of the age of the culture (1, 2). It was shown that the specific activities of ribonuclease (RNase) (EC 2.7.7.h) and acid phosphatase (APase) (EC 3.1.3.2) in crude homogenates prepared from stationary-phase cultures were consistently higher than t...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1976
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1976.tb10396.x